Evaluation of antibody-based single cell type imaging techniques coupled to multiplexed imaging of N-glycans and collagen peptides by matrix-assisted laser desorption/ionization mass spectrometry imaging

Author:

Dunne Jaclyn,Griner Jake,Romeo Martin,Macdonald Jade,Krieg Carsten,Lim Mark,Yagnik Gargey,Rothschild Kenneth J.,Drake Richard R.,Mehta Anand S.,Angel Peggi M.

Abstract

AbstractThe integration of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) with single cell spatial omics methods allows for a comprehensive investigation of single cell spatial information and matrisomal N-glycan and extracellular matrix protein imaging. Here, the performance of the antibody-directed single cell workflows coupled with MALDI-MSI are evaluated. Miralys™ photocleavable mass-tagged antibody probes (MALDI-IHC, AmberGen, Inc.), GeoMx DSP® (NanoString, Inc.), and Imaging Mass Cytometry (IMC, Standard BioTools Inc.) were used in series with MALDI-MSI of N-glycans and extracellular matrix peptides on formalin-fixed paraffin-embedded tissues. Single cell omics protocols were performed before and after MALDI-MSI. The data suggests that for each modality combination, there is an optimal order for performing both techniques on the same tissue section. An overall conclusion is that MALDI-MSI studies may be completed on the same tissue section as used for antibody-directed single cell modalities. This work increases access to combined cellular and extracellular information within the tissue microenvironment to enhance research on the pathological origins of disease. Graphical Abstract

Funder

Hollings Cancer Center, Medical University of South Carolina

National Institute of General Medical Sciences

National Institutes of Health

Publisher

Springer Science and Business Media LLC

Subject

Biochemistry,Analytical Chemistry

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