Abstract
AbstractIn order to detect the misuse of testosterone (T), urinary steroid concentrations and concentration ratios are quantified and monitored in a longitudinal manner to enable the identification of samples exhibiting atypical test results. These suspicious samples are then forwarded to isotope ratio mass spectrometry (IRMS)–based methods for confirmation. Especially concentration ratios like T over epitestosterone (E) or 5α-androstanediol over E proved to be valuable markers. Unfortunately, depending on the UGT2B17 genotype and/or the gender of the athlete, these markers may fail to provide evidence for T administrations when focusing exclusively on urine samples. In recent years, the potential of plasma steroids has been investigated and were found to be suitable to detect T administrations especially in female volunteers. A current drawback of this approach is the missing possibility to confirm that elevated steroid concentrations are solely derived from an administration of T and cannot be attributed to confounding factors. Therefore, an IRMS method for plasma steroids was developed and validated taking into account the comparably limited sample volume. As endogenous reference compounds, unconjugated cholesterol and dehydroepiandrosterone sulfate were found suitable, while androsterone and epiandrosterone (both sulfo-conjugated) were chosen as target analytes. The developed method is based on multi-dimensional gas chromatography coupled to IRMS in order to optimize the overall assay sensitivity. The approach was validated, and a reference population encompassing n = 65 males and females was investigated to calculate population-based thresholds. As proof-of-concept, samples from volunteers receiving T replacement therapies and excretion study samples were investigated.
Graphical abstract
Funder
Partnership for Clean Competition
Manfred-Donike Institute for Doping Analysis
Bundesministerium des Innern, für Bau und Heimat
Publisher
Springer Science and Business Media LLC
Subject
Biochemistry,Analytical Chemistry
Reference28 articles.
1. WADA Science/EAAS Working Group. WADA technical document – TD2021EAAS. www.wada-ama.org/sites/default/files/resources/files/td2021eaas_final_eng.pdf. Accessed 29 Mar 2021
2. Sottas PE, Baume N, Saudan C, Schweizer C, Kamber M, Saugy M. Bayesian detection of abnormal values in longitudinal biomarkers with an application to T/E ratio. Biostatistics. 2007;8:285–96.
3. Mareck U, Geyer H, Opfermann G, Thevis M, Schänzer W. Factors influencing the steroid profile in doping control analysis. J Mass Spectrom. 2008;43:877–91.
4. Kuuranne T, Saugy M, Baume N. Confounding factors and genetic polymorphism in the evaluation of individual steroid profiling. Br J Sports Med. 2014;48:848–55.
5. WADA Science / IRMS Working Group. WADA technical document – TD2021IRMS. www.wada-ama.org/sites/default/files/resources/files/td2021irms_final_eng.pdf. Accessed 29 Mar 2021
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