Validated reverse transcription droplet digital PCR serves as a higher order method for absolute quantification of Potato virus Y strains
Author:
Publisher
Springer Science and Business Media LLC
Subject
Biochemistry,Analytical Chemistry
Link
http://link.springer.com/article/10.1007/s00216-018-1053-3/fulltext.html
Reference28 articles.
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2. Dobnik D, Baebler Š, Kogovšek P, Pompe Novak M, Štebih D, Panter G, et al. [Beta]-1,3-glucanase class III promotes spread of PVYNTN and improves in planta protein production. Plant Biotechnol Rep. 2013;7(4):547–55.
3. Gutiérrez-Aguirre I, Rački N, Dreo T, Ravnikar M. Droplet digital PCR for absolute quantification of pathogens. In: Lacomme C, editor. Plant pathology: techniques and protocols. Methods in Molecular Biology. vol. 1302. New York: Springer Science+Business Media; 2015. p. 331–47.
4. Bujarsk JJ. Molecular basis of genetic variability in RNA viruses. In: Mandahar CL, editor. Molecular biology of plant viruses. Boston: Springer; 1999. p. 121–41.
5. Cankar K, Štebih D, Dreo T, Žel J, Gruden K. Critical points of DNA quantification by real-time PCR—effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol. 2006;6:37.
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