Abstract
AbstractBrachionus calyciflorus is a species complex consisting of four recently described species. Although several lines of evidence support their species status, hybridization between two of the sibling species B. calyciflorus s.s. and B. elevatus has been inferred from both field and laboratory studies. In this study, we tested for the existence of prezygotic barriers between these species by performing two types of cross-fertilization experiments. In a ‘mate competition’ experiment we exposed mictic females to equal numbers of conspecific and allospecific males and demonstrate that intraspecific fertilizations occur at much higher frequencies than interspecific fertilizations, providing evidence for a strong prezygotic reproductive barrier. This result was consistent across numerous combinations of parental genotypes. In addition, by creating interspecific combinations of mictic females and males in a ‘no choice’ experiment, we found that interspecific fertilization success is independent of fertilization direction while it does seem to depend on maternal genotype. Our results demonstrate the existence of a strong prezygotic barrier that may play an important role in the maintenance of species boundaries. Yet, the observation of hybrids also shows a potential for gene flow between the species through hybridization.
Funder
China Scholarship Council
Publisher
Springer Science and Business Media LLC
Reference78 articles.
1. Bates, D., M. Mächler, B. Bolker & S. Walker, 2014. Fitting linear mixed-effects models using lme4. arXiv preprint arXiv:14065823.
2. Behm, Jocelyn E., Anthony R. Ives & Janette W. Boughman, 2010. Breakdown in postmating isolation and the collapse of a species pair through hybridization. The American Naturalist 175(1): 11–26.
3. Carrió, E. & J. Güemes, 2014. The effectiveness of pre- and post-zygotic barriers in avoiding hybridization between two snapdragons (Antirrhinum L.: Plantaginaceae). Botanical Journal of the Linnean Society 176(2): 159–172.
4. Chin, T. A., C. E. Cáceres & M. E. Cristescu, 2019. The evolution of reproductive isolation in Daphnia. BMC Evolutionary Biology 19(1): 216.
5. Coughlan, J. M. & D. R. Matute, 2020. The importance of intrinsic postzygotic barriers throughout the speciation process. Philosophical Transactions of the Royal Society b: Biological Sciences 375(1806): 20190533.
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