Duplex real-time quantitative PCR for simultaneous detection and quantification of Pratylenchus neglectus and P. thornei

Author:

Lin Borong,Tao Ye,Wang Honghong,Liao Jinling,Zhuo Kan

Publisher

Springer Science and Business Media LLC

Subject

Horticulture,Plant Science,Agronomy and Crop Science

Reference27 articles.

1. Al-Banna, L., Ploeg, A. T., Williamson, V. M., & Kaloshian, I. (2004). Discrimination of six Pratylenchus species using PCR and species-specific primers. Journal of Nematology, 36(2), 142–146.

2. Berry, S. D., Fargette, M., Spaull, V. W., Morand, S., & Cadet, P. (2008). Detection and quantification of root-knot nematode (Meloidogyne javanica), lesion nematode (Pratylenchus zeae) and dagger nematode (Xiphinema elongatum) parasites of sugarcane using real-time PCR. Molecular and Cellular Probes, 22(3), 168–176.

3. Carrasco-Ballesteros, S., Castillo, P., Adams, B. J., & Perez-Artes, E. (2007). Identification of Pratylenchus thornei, the cereal and legume root-lesion nematode, based on SCAR-PCR and satellite DNA. European Journal of Plant Pathology, 118(2), 115–125.

4. Castillo, P., & Vovlas, N. (2007). Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, biology, pathogenicity and management. In D. J. Hunt & R. N. Perry (Eds.), Nematology monographs and perspectives 6 (p. 529). Leiden: Brill.

5. Castillo, P., Trapero-Casas, J. L., & Jiménez-Díaz, R. M. (1995). Effect of time, temperature, and inoculum density on reproduction of Pratylenchus thornei in carrot disk cultures. Journal of Nematology, 27(1), 120–124.

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