Electroporation of AsCpf1/RNP at the Zygote Stage is an Efficient Genome Editing Method to Generate Knock-Out Mice Deficient in Leukemia Inhibitory Factor-Reference-Cited by-同舟云学术

Electroporation of AsCpf1/RNP at the Zygote Stage is an Efficient Genome Editing Method to Generate Knock-Out Mice Deficient in Leukemia Inhibitory Factor

Published:2019-11-19 Issue:1 Volume:17 Page:45-53
ISSN:1738-2696
Container-title:Tissue Engineering and Regenerative Medicine
language:en
Short-container-title:Tissue Eng Regen Med

Author:

Kim Yeon Sun,Kim Gyeong Ryeong,Park Mira,Yang Seung Chel,Park So Hee,Won Ji Eun,Lee Ju Hee,Shin Ha Eun,Song Haengseok,Kim Hye-Ryun

Funder

Ministry of Education

Ministry of Health and Welfare

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Medicine (miscellaneous)

Link

http://link.springer.com/content/pdf/10.1007/s13770-019-00225-8.pdf

Reference38 articles.

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3. Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell. 2013;153:910–8.

4. Wefers B, Ortiz O, Wurst W, Kühn R. Generation of targeted mouse mutants by embryo microinjection of TALENs. Methods. 2014;69:94–101.

5. Chen YG, Forsberg MH, Khaja S, Ciecko AE, Hessner MJ, Geurts AM. Gene targeting in NOD mouse embryos using zinc-finger nucleases. Diabetes. 2014;63:68–74.

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