Abstract
AbstractSeveral isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-15N]-guanosine-5′-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.
Graphical abstract
Funder
National Institutes of Health
Publisher
Springer Science and Business Media LLC
Reference51 articles.
1. Abernathy E, Glaunsinger B (2015) Virology 479:600
2. Serganov A, Patel DJ (2007) Nat Rev Genet 8:776
3. Lau NC, Lai EC (2005) Genome Biol 6:315
4. Shang BQ, Li ML, Quan HY, Hou PF, Li ZW, Chu SF, Zheng JN, Bai J (2019) Mol Cancer 18:1
5. Schoenmaker L, Witzigmann D, Kulkarni JA, Verbeke R, Kersten GF, Jiskoot W, Crommelin DJ (2021) Int J Pharm 601:120586
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