Abstract
AbstractIn mammalian research, the implementation of the 3Rs is ubiquitous. However, the adaptation of procedures for experimental work on fish seems less extensive in comparison, even though fish model organisms are common in a broad range of research fields already. To strengthen animal welfare in experimental research, we provide an overview of current research results, including studies on the nociception of fishes. Furthermore, we describe the potential of implementing the 3Rs in fish experimental research. In the context of "Reduction", we show alternative research methods to lethal sampling. Considering "Refinement", we point out possibilities to improve fish handling and indicate that adaptations to the individual species ecology are necessary. Under the aspect of "Replacement", we describe the high potential of cell cultures that can be obtained from fish tissue and give an overview of the already extensive use in ecotoxicology and virology. In addition, we illustrate that cell cultures could also be increasingly used for basic research.
Graphical abstract
Funder
Leibniz-Institut für Nutztierbiologie
Forschungsinstitut für Nutztierbiologie (FBN)
Publisher
Springer Science and Business Media LLC
Reference191 articles.
1. Ahne W (2007) Tierversuche, im spannungsfeld von praxis und bioethik, 1st edn. Klett-Cotta Verlag J G Cotta’sche Buchhandlung Nachfolger GmbH, Stuttgart, Germany, p 21
2. Ahne W, Halder M (1990) The use of the R1-fish cell culture for detection of toxicity of waste water according to the German Waste Water Act. Altex 7:17–26
3. Alvaro R, Rico A, Rabuñal J, Gestal M (2017) Fish tracking with computer vision techniques: an application to vertical slot fishways. In: Multi-core computer vision and image processing for intelligent applications ISBN: 9781522508908, pp 74–105.
4. American Fisheries Society (2014) Guidelines for the use of fishes in research. available online: https://fisheriesorg/docs/wp/Guidelines-for-Use-of-Fishes.pdf, page 20–21. (Accesses on 17.06.2022)
5. Arnegard ME, Carlson BA (2005) Electric organ discharge patterns during group hunting by a mormyrid fish. Proc Biol Sci 272:1305–1314. https://doi.org/10.1098/rspB2005.3101
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