DNA degrades during storage in formalin-fixed and paraffin-embedded tissue blocks

Author:

Guyard Alice,Boyez Alice,Pujals Anaïs,Robe Cyrielle,Tran Van Nhieu Jeanne,Allory Yves,Moroch Julien,Georges Odette,Fournet Jean-Christophe,Zafrani Elie-Serge,Leroy Karen

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,General Medicine,Pathology and Forensic Medicine

Reference23 articles.

1. Ferrer I, Armstrong J, Capellari S, Parchi P, Arzberger T, Bell J, Budka H, Strobel T, Giaccone G, Rossi G, Bogdanovic N, Fakai P, Schmitt A, Riederers P, Al-Sarraj S, Ravid R, Kretzschmar H (2007) Effects of formalin fixation, paraffin embedding, and time of storage on DNA preservation in brain tissue: a BrainNet Europe study. Brain Pathol 17(3):297–303. doi: 10.1111/j.1750-3639.2007.00073.x

2. Hedegaard J, Thorsen K, Lund MK, Hein AM, Hamilton-Dutoit SJ, Vang S, Nordentoft I, Birkenkamp-Demtroder K, Kruhoffer M, Hager H, Knudsen B, Andersen CL, Sorensen KD, Pedersen JS, Orntoft TF, Dyrskjot L (2014) Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue. PLoS One 9(5):e98187. doi: 10.1371/journal.pone.0098187

3. Kokkat TJ, Patel MS, McGarvey D, LiVolsi VA, Baloch ZW (2013) Archived formalin-fixed paraffin-embedded (FFPE) blocks: a valuable underexploited resource for extraction of DNA, RNA, and protein. Biopreserv Biobank 11(2):101–106. doi: 10.1089/bio.2012.0052

4. Nam SK, Im J, Kwak Y, Han N, Nam KH, Seo AN, Lee HS (2014) Effects of fixation and storage of human tissue samples on nucleic acid preservation. Korean J Pathol 48(1):36–42. doi: 10.4132/KoreanJPathol.2014.48.1.36

5. Bhat S, Curach N, Mostyn T, Bains GS, Griffiths KR, Emslie KR (2010) Comparison of methods for accurate quantification of DNA mass concentration with traceability to the international system of units. Anal Chem 82(17):7185–7192. doi: 10.1021/ac100845m

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