Vasoactive intestinal peptide (VIP) binding to solubilized material from rat liver plasma membranes-Reference-Cited by-同舟云学术

Vasoactive intestinal peptide (VIP) binding to solubilized material from rat liver plasma membranes

Published:1986-01-01 Issue:1 Volume:6 Page:39-44
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Guerrero J. M.¹,Calvo J. R.¹,Garrido M. R.¹,Molinero P.¹,Osuna C.¹,Jiménez J.¹,Goberna R.¹

Affiliation:

1. Department of Biochemistry, School of Medicine, Avda. Sánchez Pizjuan, 4, 41009-Sevilla, Spain

Abstract

A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubitized proteins bind specifically [125I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of detergent (below 0.001%) and is inhibited by native peptide. A molecular weight of about 80,000 was estimated for VIP-binding proteins by reference to a series of globular markers of proteins. Binding to VIP soluble proteins is specific and dependent on time as studied by the Hummel and Dreyer (Biochim. Biophys. Acta63:530–532, 1962) assay.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/6/1/39/519693/bsr0060039.pdf

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2. Molecular Analysis of Vasoactive Intestinal Peptide Receptors: A Comparison with Receptors for VIP-Related Peptides;Annals of the New York Academy of Sciences;1988-06