Abstract
Abstract
In order to achieve growth in agricultural production, nitrogen fertilisers are widely used. The urease enzyme, present in soil, vastly accelerates the pace of nitrogen transformation into plant-available forms. Rapid acceleration causes high nitrogen losses and the products of said reactions (e.g., greenhouse gases) which are released into the environment (Hube et al., Agric Ecosyst Environ: 46–54, 2017). Many countries have imposed regulations and laws regarding the compulsory use of urease inhibitory precautions when fertilising with urea. However, the inhibitors in use involve a high cost and new environmental risks. In this study, watercress (Nasturium officinale) is presented and investigated as an alternative. Ultraviolet-visible spectroscopy (UV-Vis) Han’s method, high-performance liquid chromatography mass spectrometry (HPLC-MS) and proton nuclear magnetic resonance (1H-NMR). An organosulfur compound dimethyl thiosulfinate (DMTS) was recognised in watercress extract, DMTS concentration was assessed and its inhibitory influence on native and soil ureases confirmed. The urease inhibition was of competitive character. Watercress have potential use as a natural source of urease inhibitor both in agriculture and other branches.
Graphical Abstract
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Soil Science,Agronomy and Crop Science
Reference43 articles.
1. Agelopoulos NG, Keller MA (1994) Plant-natural enemy association in tritrophic system, Cotesia rubecula-Pieris rapae-Brassicaceae (Cruciferae). III: collection and identification of plant and frass volatiles. J Chem Ecol:1955–1967. https://doi.org/10.1007/BF02066236
2. Biglar M, Soltani K, Nabati F, Bazl R, Mojab F, Amanlou M (2012) A preliminary investigation of the jack-bean urease inhibition by randomly selected traditionally used herbal medicine. IJPR 1(3):831–837
3. Boyd DR, Sharma ND, Stepherd SD, Allenmark SG, Allen C (2014) Enzyme-catalysed oxidation of 1,2-disulfides to yield chiral thiosulfinate, sulfoxide and cis-dihydrodiol metabolites. RSC Adv. https://doi.org/10.1039/C4RA02923B
4. Byrne MP, Tobin JT, Forrestal PJ, Danaher M, Nkwonta CG, Richards K, Cummins E, Hogan SA, O’Callaghan TF (2020) Urease and nitrification inhibitors—as mitigation tools for greenhouse gas emissions in sustainable dairy systems: a review. Sustainability:6018. https://doi.org/10.3390/su12156018
5. Callahan BP, Yuan Y, Wolfenden R (2005) The burden borne by urease. J Am Chem Soc:10828–10829. https://doi.org/10.1021/ja0525399