Cloning and expression of staphylokinase-streptokinase recombinant protein in E. coli BL21(DE3)

Author:

Buniya Harith K.ORCID,Hameed Almuthana K.,Al-Hayawi Anas Y.

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Plant Science,Genetics,Molecular Biology,Animal Science and Zoology,Biochemistry,Ecology, Evolution, Behavior and Systematics

Reference16 articles.

1. Assiri AS, El-Gamal BA, Hafez EE, Haidara MA (2014) Production of recombinant streptokinase from Streptococcus pyogenes isolate and its potential for thrombolytic therapy. Saudi Med J 35:1482–1488

2. Banerjee A, Chisti Y, Banerjee UC (2004) Streptokinase—a clinically useful thrombolytic agent. Biotechnol adv 22:287–307. DOI: https://doi.org/10.1016/j.biotechadv.2003.09.004

3. Buniya HK, Murugan V, Thangadurai C (2014) Cloning and expression of hybrid streptokinase towards clot-specific activity. J Microbiol methods 98:84–88. DOI: https://doi.org/10.1016/j.mimet.2014.01.006

4. Buniya HK, Farhan HA (2016) Cloning and Expression of Staphylokinase gene produced from Staphylococcus aureus in E. coli DH5α. Al-Anbar J Vet Sci 9: 7–14

5. Collen D (1998) Staphylokinase: a potent, uniquely fibrin-selective thrombolytic agent. Nat Med 4:279–284. DOI: https://doi.org/10.1038/nm0398-279

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