Separation of human endothelial cells from fibroblasts by centrifugation in Percoll gradients-Reference-Cited by-同舟云学术

Separation of human endothelial cells from fibroblasts by centrifugation in Percoll gradients

Published:1985-06-01 Issue:6 Volume:5 Page:469-472
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Sbarbati Rosella¹

Affiliation:

1. C.N.R. Institute of Clinical Physiology, Via Savi 8, 56100 Pisa, Italy

Abstract

Human endothelial cells from the umbilical vein and skin fibroblasts can be separated by means of centrifugation in a density gradient of Percoll. Cells show a good recovery in culture. Viability is not impaired.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/5/6/469/471428/bsr0050469.pdf

Reference4 articles.

1. Pertoft H & Laurent TC (1982) Isopycnic separation of cells by centrifugation in Percoll gradients. In: Cell Function and Differentiation, Part A: 95, Alan R. Liss, New York.

2. Sbarbati R (1984) Methods of separation and culture of microvascular endothelial cells from human skin. Proceedings of the 22nd Annual Meeting of the Cell, Tissue, Organ Culture (C.T.O.C.) Study Group: Noordwijkerhout (NL), Rep. T.N.O., Rijswijk (NL).

3. Stel HV, Sakariassen KS, Scholte BJ, Veerman ECI, van der Kwast ThH, de Groot PhG, Sixma JJ & van Mourik JA (1984) Characterization of 25 monoclonal antibodies to factor VIII-von Willebrand factor: Relationship between ristocetin-induced platelet aggregation and platelet adherence to subendothelium. Blood63, 1408?1415.

4. Willems Ch, Astaldi GCB, de Groot PhG, Jansson MC, Gonsalvez MD, Zeijlemaker WP, van Mourik JA & van Aken WG (1982) Media conditioned by cultured human vascular endothelial cells inhibit the growth of vascular smooth muscle cells. Exp. Cell Res.139, 191?197.

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