Abstract
Abstract
Dracocephalum moldavica L. is an aromatic plant emitting intense lemon scent. The aboveground parts of the plants constitute raw material for medicine and food industry. In contrast to the comprehensively investigated trichomes, there are only few studies of the histochemical characteristics of the leaves of essential oil-bearing plants from the family Lamiaceae. The present study shows the micromorphology, anatomy, and histochemistry of the leaves of the analysed species. The research aimed to determine the location of essential oil and other specialised metabolites in leaf tissues. The investigations of fresh and fixed material were carried out with the use of light, fluorescence, and scanning electron microscopy. Additionally, the content and composition of essential oil in the leaves were determined with the GC/MS method. The leaf epidermis had non-glandular unbranched trichomes and three types of glandular trichomes: peltate as well as long and short capitate trichomes. The results of the histochemical assays showed positive reactions to lipids and to some secondary compounds such as essential oil, terpenes, phenolic compounds, and flavonoids in all types of the glandular and non-glandular trichomes. The same compounds were found in the epidermis cells of the leaves. The results of the present study indicate that the intense smell of the leaves is associated with emission of essential oil not only by the glandular and non-glandular trichomes but also by the leaf epidermis cells. The main components in the essential oil (0.10%) include geranial, neral, geraniol, nerol, and trans-myrtanol acetate. Since D. moldavica has been used as an adulterant of Melissa officinalis L., the anatomical traits of leaves and the essential oil composition in both species were compared in the study.
Funder
Ministerstwo Nauki i Szkolnictwa Wyższego
Publisher
Springer Science and Business Media LLC
Reference86 articles.
1. Abdellatif F, Boudjella H, Zitouni A, Hassani A (2014) Chemical composition and antimicrobial activity of the essential oil from leaves of Algerian Melissa officinalis L. EXCLI J 13:772–781
2. Adams RP (2001) Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy, 3rd edn. Allured Publishing Corporation, Carol Stream
3. Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectroscopy, 4th edn. Allured Publishing Corporation, Carol Stream
4. Amin G (1991) Popular medicinal plants of Iran, vol 1. Deputy of Research, Ministry of Health and Educational Medicine, Tehran
5. Aprotosoaie AC, Mihai CT, Vochita G, Rotinberg P, Trifan A, Luca SV, Petreus T, Gille E, Miron A (2016) Antigenotoxic and antioxidant activities of a polyphenolic extract from European Dracocephalum moldavica L. Ind Crop Prod 79:248–257.
https://doi.org/10.1016/j.indcrop.2015.11.004
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