Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells

Author:

Chotigeat W.,Watanapokasin Y.,Mahler S.,Gray P. P.

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Clinical Biochemistry,Biomedical Engineering,Bioengineering,Biotechnology

Reference11 articles.

1. Chappel SC, Ulloa-Aguirre A, Coutifaris C (1983) Biosynthesis and secretion of FSH. Endo. Rev. 4: 179?211.

2. CHU SW and Walker WA (1986) Developmental changes in the activities of sialyl and fucosyltransferases in rat small intestine. Biochem. Biophys. Acta. 883: 496?500.

3. Gebert C and Gray PP (1994) Expression of FSH in CHO cells I. Comparison of promoter types and effects of their respective inducers. Cytotechnology 14: 39?45.

4. Goochee CF and Monica T (1990) Environmental effects on protein glycosylation. Bio/Technology 8: 421?427.

5. Gray PP, Crowley JM and Marsden WL (1990) Growth of a recombinant CHO cell line and high level expression in protein free medium. In: Murakami H (ed.) Trends in Animal Cell Technology, VCH I Kodansha, Tokyo: 265?270.

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