Approaches for recombinant human factor IX production in serum-free suspension cultures

Author:

do Amaral Robson Luis Ferraz,de Sousa Bomfim Aline,de Abreu-Neto Mário Soares,Picanço-Castro Virgínia,de Sousa Russo Elisa Maria,Covas Dimas Tadeu,Swiech Kamilla

Funder

FAPESP

Publisher

Springer Science and Business Media LLC

Subject

General Medicine,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology

Reference31 articles.

1. Altamiro C, Berrios J, Vergara M, Becerra S (2013) Advances in improving mammalian cells metabolism for recombinant protein production. Eur J Biotechnol 16:1–14

2. Blostein M, Cuerquis J, Landry S, Galipeau J (2008) The carboxylation efficiency of the vitamin K-dependent clotting factors studies with factor IX. Haemophilia 14:1063–1068

3. Bomfim A (2013) Cloning and expression of recombinant factor IX in 293T and SK-Hep-1 cells and characterization of producing cells. Master Dissertation, School of Pharmaceutical Sciences of Ribeirão Preto, University of Sao Paulo, Brazil). http://www.teses.usp.br/teses/disponiveis/60/60135/tde-13122013-111826/en.php

4. Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G (2010) Serum-free cell culture the serum-free media interative online database. ALTEX 27:53–62

5. Cervera L, Gutierrez-Granados S, Martinez M, Blanco J, Godia F, Segura M (2013) Generation of HIV-1 Gag VLPs by transient transfection of HEK 293 suspension cell cultures using an optimized animal-derived component free medium. J Biotechnol 166:152–165

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