Engineering a synthesis-friendly serum responsive promoter for antibiotic selection of genomic integration in cell-based therapy
Author:
Funder
Natural Sciences and Engineering Research Council of Canada
Institute of Aging
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s10529-022-03244-z.pdf
Reference20 articles.
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2. Ede C, Chen X, Lin M-Y, Chen YY (2016) Quantitative analyses of core promoters enable precise engineering of regulated gene expression in mammalian cells. ACS Synth Biol 5:395–404. https://doi.org/10.1021/acssynbio.5b00266
3. Fan L, Kadura I, Krebs LE et al (2012) Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells. Biotechnol Bioeng 109:1007–1015. https://doi.org/10.1002/bit.24365
4. Gibson DG, Young L, Chuang RY et al (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6:343–345. https://doi.org/10.1038/nmeth.1318
5. Gossen M, Bujard H (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 89:5547–5551. https://doi.org/10.1073/pnas.89.12.5547
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