Helicase translocation assay method using avidin and biotinylated nucleotides
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Biotechnology,Bioengineering,Applied Microbiology and Biotechnology
Link
http://link.springer.com/content/pdf/10.1007/s10529-008-9781-7.pdf
Reference13 articles.
1. Byrd AK, Raney KD (2004) Protein displacement by an assembly of helicase molecules aligned along single-stranded DNA. Nat Struct Mol Biol 11:531–538
2. Delagoutte E, von Hippel PH (2003) Helicase mechanisms and the coupling of helicases within macromolecular machines. Part II: integration of helicases into cellular processes. Q Rev Biophys 36:1–69
3. Green NM (1990) Avidin and streptavidin. Methods Enzymol 184:51–67
4. Haugland GT, Shin JH, Birkeland NK et al (2006) Stimulation of MCM helicase activity by a Cdc6 protein in the archaeon Thermoplasma acidophilum. Nucleic Acids Res 34:6337–6344
5. Holmberg A, Blomstergren A, Nord O et al (2005) The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures. Electrophoresis 26:501–510
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