Decoding Selection Bias Imparted by Unpaired Cysteines: a Tug of War Between Expression and Affinity
Author:
Funder
Science Foundation Ireland
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,General Medicine,Bioengineering,Biotechnology
Link
https://link.springer.com/article/10.1007/s12010-017-2691-1/fulltext.html
Reference26 articles.
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2. Albrecht, H., DeNardo, G. L., & DeNardo, S. J. (2006). Monospecific bivalent scFv-SH: Effects of linker length and location of an engineered cysteine on production, antigen binding activity and free SH accessibility. Journal of Immunological Methods, 310(1-2), 100–116. https://doi.org/10.1016/j.jim.2005.12.012.
3. Ayyar, B. V., Hearty, S., & O’Kennedy, R. (2015). Facile domain rearrangement abrogates expression recalcitrance in a rabbit scFv. Applied Microbiology and Biotechnology, 99(6), 2693–2703. https://doi.org/10.1007/s00253-014-6268-4.
4. Chi, X. S., Landt, Y., Crimmins, D. L., Dieckgraefe, B. K., & Ladenson, J. H. (2002). Isolation and characterization of rabbit single chain antibodies to human Reg Iα protein. Journal of Immunological Methods, 266(1-2), 197–207. https://doi.org/10.1016/S0022-1759(02)00117-5.
5. Ewert, S., Huber, T., Honegger, A., & Plückthun, A. (2003). Biophysical properties of human antibody variable domains. Journal of Molecular Biology, 325(3), 531–553. https://doi.org/10.1016/S0022-2836(02)01237-8.
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