Combining E-ice-COLD-PCR and Pyrosequencing with Di-Base Addition (PDBA) Enables Sensitive Detection of Low-Abundance Mutations
Author:
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,General Medicine,Bioengineering,Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s12010-023-04718-0.pdf
Reference33 articles.
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3. Chubarov, A. S., Oscorbin, I. P., Filipenko, M. L., Lomzov, A. A., & Pyshnyi, D. V. (2020). Allele-specific PCR for KRAS mutation detection using phosphoryl guanidine modified primers. Diagnostics (Basel), 10, 872–886.
4. Machnicki, M. M., Glodkowska-Mrowka, E., Lewandowski, T., Ploski, R., Wlodarski, P., & Stoklosa, T. (2013). ARMS-PCR for detection of BRAF V600E hotspot mutation in comparison with Real-Time PCR-based techniques. Acta Biochimica Polonica, 60, 57–64.
5. Yamada, N., Oizumi, S., Asahina, H., Shinagawa, N., Kikuchi, E., Kikuchi, J., Sakakibara-Konishi, J., Tanaka, T., Kobayashi, K., Hagiwara, K., & Nishimura, M. (2012). The peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp-based test for epidermal growth factor receptor mutations in bronchoscopic cytological specimens of non-small cell lung cancer. Oncology, 82, 341–346.
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