Development of an In Situ Detachment Protocol of Vero Cells Grown on Cytodex1 Microcarriers Under Animal Component-Free Conditions in Stirred Bioreactor

Author:

Rourou Samia,Riahi Nesrine,Majoul Samy,Trabelsi Khaled,Kallel Héla

Publisher

Springer Science and Business Media LLC

Subject

Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,General Medicine,Bioengineering,Biotechnology

Reference33 articles.

1. Birch, J. R. (1999). Suspension culture of animal cells. In M. C. Flickinger & S. W. Drew (Eds.), Encyclopedia of bioprocess technology: fermentation, biocatalysis, and bioseparation (pp. 2509–2516). New York: Wiley.

2. Shao, M., Lei, J., Wei, C., & Ouyang, F. (2002). Analysis of Vero cell growth behaviour on microcarriers by means of environmental scanning electron microscopy. Science in China (Series C), 45, 149–158.

3. Montagnon, B. J., Fanger, B., & Nicolas, A. J. (1981). The large-scale cultivation of Vero cells in microcarrier culture for virus vaccine production: preliminary results for killed poliovirus vaccine. Development in Biological Standardization, 47, 55–64.

4. Van Wezel, A. L. (1967). Growth of cell strains and primary cells on microcarriers in homogeneous culture. Nature, 216, 64–65.

5. Wang, Y., & Ouyang, F. (1999). Bead-to-bead transfer of Vero cells in microcarrier culture. Cytotechnology, 31, 221–224.

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