Isolation and Characterization of Cross-Neutralizing Human Anti-V3 Single-Chain Variable Fragments (scFvs) Against HIV-1 from an Antigen Preselected Phage Library

Author:

Kumar Rajesh,Kumari Ruchi,Khan Lubina,Sankhyan Anurag,Parray Hilal Ahmad,Tiwari Ashutosh,Wig Naveet,Sinha Subrata,Luthra Kalpana

Funder

Department of Biotechnology , Ministry of Science and Technology

Publisher

Springer Science and Business Media LLC

Subject

Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,General Medicine,Bioengineering,Biotechnology

Reference38 articles.

1. Zolla-Pazner, S., Zhong, P., Revesz, K., Volsky, B., Williams, C., Nyambi, P., & Gorny, M. K. (2004). The cross-clade neutralizing activity of a human monoclonal antibody is determined by the GPGR V3 motif of HIV type 1. AIDS Research and Human Retroviruses, 20(11), 1254–1258.

2. Zolla-Pazner, S., Cohen, S. S., Krachmarov, C., Wang, S., Pinter, A., & Lu, S. (2008). Focusing the immune response on the V3 loop, a neutralizing epitope of the HIV-1 gp120 envelope. Virology, 372(2), 233–246.

3. Gorny, M. K., VanCott, T. C., Hioe, C., Israel, Z. R., Michael, N. L., Conley, A. J., et al. (1997). Human monoclonal antibodies to the V3 loop of HIV-1 with intra- and interclade cross-reactivity. Journal of Immunology (Baltimore, Md: 1950), 159(10), 5114–5122.

4. Gorny, M. K., Williams, C., Volsky, B., Revesz, K., Cohen, S., Polonis, V. R., Honnen, W. J., Kayman, S. C., Krachmarov, C., Pinter, A., & Zolla-Pazner, S. (2002). Human monoclonal antibodies specific for conformation-sensitive epitopes of V3 neutralize human immunodeficiency virus type 1 primary isolates from various clades. Journal of Virology, 76(18), 9035–9045.

5. Gorny, M. K., Williams, C., Volsky, B., Revesz, K., Wang, X. H., Burda, S., Kimura, T., Konings, F. A. J., Nadas, A., Anyangwe, C. A., Nyambi, P., Krachmarov, C., Pinter, A., & Zolla-Pazner, S. (2006). Cross-clade neutralizing activity of human anti-V3 monoclonal antibodies derived from the cells of individuals infected with non-B clades of human immunodeficiency virus type 1. Journal of Virology., 80(14), 6865–6872.

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