Abstract
Abstract
In the current study, a significant amount of ulvan was extracted from Ulva lactuca collected from Alexandria coastline, Egypt, using a simple extraction method. According to the chemical analysis, the obtained polysaccharide content is estimated to be 36.50 g/100 g with a high sulfate content of 19.72%. Physio-chemically, the FTIR analysis confirmed the presence of sulfated groups attached to the carbohydrate backbone. The GC–MS results revealed the presence of various monosaccharides with relative abundances in the order: fucopyranose (22.09%) > L-rhamnose (18.17%) > L-fucose (17.46%) > rhamnopyranose (14.29%) > mannopyranose (8.59%) > α-D-glactopyranose (7.64%) > galactopyranose (6.14%) > β-arabinopyranose (5.62%). In addition, the SEM–EDX depicted an amorphous architecture with a majority wt% for the elements of C, O, and S. The partially purified ulvan demonstrated potent antimicrobial activity against some fish and human pathogenic microbes. The inhibition zone diameter ranged from 11 to 18 mm. On the other hand, the prepared ulvan-chitosan hydrogel significantly improved the antimicrobial activity as the inhibition zone diameter ranged from 12 to 20. Moreover, when compared to the controls, the extracted ulvan demonstrated anti-fouling properties and successfully disrupted the biofilm formed on a glass slide submerged in seawater.
Funder
National Institute of Oceanography & Fisheries
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,General Medicine,Bioengineering,Biotechnology
Reference75 articles.
1. Babu, R. P., O’Connor, K., & Seeram, R. (2013). Current progress on bio-based polymers and their future trends. Progress in Biomaterials, 2, 1–16.
2. Masakuni, T., Tamanaha, M., Tamashiro, Y., & Uechi, S. (2015). Structure of ulvan isolated from the edible green seaweed, Ulva pertusa. Advances in Bioscience and Biotechnology, 6, 645–655.
3. Baky, H. H. A. E., & El-Baroty, G. S. (2013). Healthy benefit of microalgal bioactive substances. Journal of Aquatic Sciences, 1, 11–22.
4. Cunha, L., & Grenha, A. (2016). Sulfated seaweed polysaccharides as multifunctional materials in drug delivery applications. Marine Drugs, 14, 1–41.
5. Lahaye, M., & Robic, A. (2007). Structure and functional properties of ulvan, a polysaccharide from green seaweeds. Biomacromolecules, 8, 1765–1774.
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