Decontamination of DNA in Taq DNA polymerase reagents using nylon membranes for monitoring of GMOs
Author:
Funder
Ministry of Trade, Industry and Energy
Ministry of Education
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s11816-021-00723-z.pdf
Reference25 articles.
1. Beckers T, Schmidt P, Hilgard P (1994) Highly sensitive northern hybridization of rare mRNA using a positively charged nylon membrane. Biotechniques 16(6):1074–1078
2. Böttger EC (1990) Frequent contamination of Taq polymerase with DNA. Clin Chem 36(6):1258–1259. https://doi.org/10.1093/clinchem/36.6.1258b
3. Carroll NM, Adamson P, Okhravi N (1999) Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion. J Clin Microbiol 37(10):3402–3404. https://doi.org/10.1128/JCM.37.10.3402-3404.1999
4. Champlot S, Berthelot C, Pruvost M, Bennett EA, Grange T, Geigl E-M (2010) An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications. PLoS ONE 5(9):e13042. https://doi.org/10.1371/journal.pone.0013042
5. Chiang CS, Liu CP, Weng LC, Wang NY, Liaw GJ (2005) Presence of beta-lactamase gene TEM-1 DNA sequence in commercial Taq DNA polymerase. J Clin Microbiol 43(1):530–531. https://doi.org/10.1128/JCM.43.1.530-531.2005
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