Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera
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Published:2024-02-21
Issue:5
Volume:250
Page:1401-1416
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ISSN:1438-2377
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Container-title:European Food Research and Technology
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language:en
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Short-container-title:Eur Food Res Technol
Author:
Muthu Sakthivel,Gopal Venkateshbabu,AL-Younis Zena Kadhim,Altemimi Ammar B.,Perumal Palani,Lakshmikanthan Mythileeswari,Krishnan Kathiravan,Najm Mazin A. A.,Giuffrè Angelo Maria,Govindan Lakshmanan
Abstract
AbstractCalotropis procera produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of C. procera has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in C. procera plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg−1 as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca2+ and Mg2+ were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both Escherichia coli (MICs 14 µg ml−1) and Bacillus cereus (MICs 13 µg ml−1). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both E. coli and B. cereus. Therefore, the LL obtained from C. procera maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries.
Graphical abstract
Funder
Università degli Studi Mediterranea di Reggio Calabria
Publisher
Springer Science and Business Media LLC
Reference60 articles.
1. Hua J, Liu Y, Xiao CJ, Jing SX, Luo SH, Li SH (2017) Phytochemistry 136:56
2. Gracz-bernaciak J, Mazur O, Nawrot R (2021) Int J Mol Sci 22:12427
3. Salomé Abarca LF, Klinkhamer PGL, Choi YH (2019) Planta Med 85:856
4. Agrawal AA, Hastings AP (2019) J Chem Ecol 45:1004
5. Ozber N, Carr SC, Morris JS, Liang S, Watkins JL, Caldo KM, Hagel JM, Ng KKS, Facchini PJ (2022) Nat Commun 13:1