Multiplex real-time PCR for the simultaneous detection and quantification of DNA from three transgenic rice species and construction and application of an artificial oligonucleotide as reference molecule

Author:

Köppel René,Zimmerli Franziska,Breitenmoser Alda

Publisher

Springer Science and Business Media LLC

Subject

Industrial and Manufacturing Engineering,Biochemistry,General Chemistry,Food Science,Biotechnology

Reference17 articles.

1. Yang L et al (2005) Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR. Plant Cell Rep 23:10–11

2. Prior FA, Tackaberry ES, Aubin RA, Casley WL (2006) Accurate determination of zygosity in transgenic rice by real-time PCR does not require standard curves or efficiency correction. Transgenic Res 15:261–265

3. Grain testing method for the detection of rice GM event LLRice601 using RT-PCR protocols PGS0505 and PGS0476 (2006) Molecular and Biochemical Analytical Services, Bayer BioScience N.V., Belgium

4. Report on the Verification of a Construct-Specific Detection Method for Identification of GM-Events Containing P35SBAR using a Real-time PCR Assay, European Commission JRC

5. Event-specific method for the quantification of rice line LLRice62 using real-time PCR, European Commission JRC. http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm

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