Design of a liposome with the property to induce CD16 on cervical cancer cells with a purified spermidine-cholesterol

Abstract

AbstractThe purpose of the study is to investigate the use of a purified cationic lipid for fabricate liposomes capable of inducing the appearance of CD16 receptors on the membranes of cervical carcinoma cells. The methods used in this study are chemical synthesis of purified spermidine-cholesterol, liposome fabrication, UPLC-MS analysis, cell culture, and flow cytometry. This study reveals the construction of a liposomal system with a highly purified spermidine-cholesterol with CD16 inducing capacity on cervical cancer cells. We were able to synthetize a highly purified spermidine-cholesterol to fabricate a liposomal system capable to induce CD16 on cervical cancer cells.