Simultaneous ultrastructural identification of growth hormone and its messenger ribonucleic acid using combined immunohistochemistry and non-radioisotopicin situ hybridization: A technical note

Author:

Matsuno Akira,Utsunomiya Hirotoshi,Ohsugi Yoshitaka,Takekoshi Susumu,Sanno Naoko,Yoshiyuki Osamura R.,Nagao Koichi,Tamura Akira,Nagashima Tadashi

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Anatomy

Reference22 articles.

1. Egger, D., Troxier, M. &Bienz, K. (1994) Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry.J. Histochem. Cytochem. 42, 815–22.

2. Escaig-Haye, F., Grigogiev, V., Sharova, I., Rudneva, V., Buckrinskaya, A. &Fournier, J.G. (1992) Ultrastructural localization of HIV-1 RNA and core proteins. Simultaneous visualization using double immunogold labelling after in situ hybridization and immunocytochemistry.J. Submicrosc. Cytol. Pathol. 24, 437–43.

3. Gingras, D. &Bendayan, M. (1995) Colloidal gold electron microscopic in situ hybridization: combination with immunocytochemistry for the study of insulin and amylase secretion.Cell Vision 2, 218–25.

4. Guitteny, A.F. &Bloch, B. (1989) Ultrastructural detection of the vasopressin messenger RNA in the normal and Brattleboro rat.Histochemistry,92, 277–81.

5. Jirikowski, G.F., Sanna, P.P. &Bloom, F.E. (1990) mRNA coding for oxytocin is present in axons of the hypothalamo-neurohypophyseal tract.Proc. Natl. Acad. Sci. USA 87, 7400–4.

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