Integration of small RNAs, degradome, and transcriptome sequencing provides insights into the differences between Shizhu ginseng and Yuan ginseng
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Published:2020-10-30
Issue:
Volume:
Page:
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ISSN:0971-7811
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Container-title:Journal of Plant Biochemistry and Biotechnology
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language:en
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Short-container-title:J. Plant Biochem. Biotechnol.
Author:
Peng Mengyuan,Chen Yanlin,Wang Wenjuan,He Zhihua,Dai Wangqiang,Lin Zhiyun,Yang Zemin,Gong Mengjuan,Yin Yongqin,Han Bin,Zeng Yu,Wang Yingfang
Abstract
Abstract
Panax ginseng is one of the most popular herbs which have been used as an important traditional Chinese medicine since ancient times. Yuan ginseng and Shizhu ginseng,which belong to P. ginseng, are widely used as substitutes for wild ginseng in clinical practice. Clinical practice has proved that the clinical efficacy of Shizhu ginseng is better than Yuan ginseng. However, current research cannot completely explain this phenomenon. Considering that small RNA may be one of the pharmacodynamic substances of P. ginseng, it is challenging to investigate differential miRNAs between Shizhu ginseng and Yuan ginseng. In this study, the transcriptome, small RNAome and degradome of P. ginseng were studied by high-throughput sequencing. A total of 63,875 unigenes and 43,950,137 small RNA clean reads were obtained from the roots of P. ginseng. Among 3206 differentially expressed genes, 1190 genes were up-regulated in Yuan ginseng when compared with Shizhu ginseng. 24 known differential miRNAs and 7 novel differential miRNAs were obtained. The 304 targets of 24 differentially expressed miRNA (17 known and 7 novel) families are mainly related to energy metabolism, biotic stress and disease immunity in ginseng itself. Through the association analysis of mRNA and miRNA, our work gives a better understanding of the difference between Yuan ginseng and Shizhu ginseng. Considering the cross-kingdom regulation of plant miRNAs, our results may provide a foundation for understanding the miRNA-dependent clinical efficacy in P. ginseng.
Funder
Young Scientists Fund Natural Science Foundation of Guangdong Province
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Agronomy and Crop Science,Biotechnology
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