Author:
Zhao Le,Yan Hang,Zhu Yun-hao,Jia Hao-yu,Han Yong-guang,Zheng Xiao-ke,Wang Min,Feng Wei-sheng
Abstract
AbstractAs a commonly used traditional Chinese medicine, Descurainia sophia has various reported pharmacological activities such as prevention of chronic diseases, cardiovascular protection, neuroprotection, anticancer, antioxidant, and has high nutritional values, but its mitochondrial genome (mitogenome) has not been reported. In this study, the D. sophia mitogenome was sequenced, assembled and annotated using next generation sequencing technologies on the Illumina HiSeq and PacBio Sequel platform. The results showed that the D. sophia mitogenome was a circular DNA molecule with the length of 265,457 bp and the GC content of 44.78%, encoding unique 56 genes, including 35 protein coding genes, 18 tRNA genes and 3 rRNA genes. We analyzed codon preference, repeated sequences, DNA sequence transformation, phylogenetic relationship, RNA editing and synteny in the D. sophia mitogenome. Based on the DNA sequences of mitogenomes, the phylogenetic and synteny analysis indicated that D. sophia was more closely related to Boechera stricta, and the mitogenome of D. sophia underwent frequent genome recombination. In this study, we obtained the complete mitogenome of D. sophia, which can provide valuable data support for future studies on genetic characteristics, phylogenetic relationships, species identification and development of new molecular markers in D. sophia.
Funder
Training Program for Young Teachers in Colleges and Universities of Henan Province
National Key Research and Development Project
Henan Province High-Level Personnel Special Support “ZhongYuan One Thousand People Plan”
Major Science and Technology Project in Henan Province
Publisher
Springer Science and Business Media LLC
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