Rapid green synthesis of non-cytotoxic silver nanoparticles using aqueous extracts of 'Golden Delicious' apple pulp and cumin seeds with antibacterial and antioxidant activity

Abstract

Abstract A simple, facile and rapid microwave irradiated system was applied to synthesize silver nanoparticles using 'Golden Delicious' apple pulp (Malus domestica) and cumin (Cuminum cyminum) seed extracts. The phytosynthesized AgNPs were characterized by Ultraviolet–Visible Spectroscopy (UV–vis), Fourier transform infrared (FTIR), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM) and Zeta sizer analysis. In the study, the presence of face-centered cubic crystalline structured metallic silver in AgNPs from apple and cumin extracts and the monodisperse nature of AgNPs with the size distribution range of 5.46–20 nm and 1.84–20.57 nm were confirmed, respectively. This study established an efficient green synthesis approach that created so far, the smallest silver nanoparticles by using these two extracts. According to the results obtained, AgNPs synthesized using both extracts were non-toxic against L929 mouse fibroblast cells, while they were effective against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria with a greater effect on S. aureus. Moreover, AgNPs synthesized through cumin extract exhibited a higher ABTS scavenging ability (96.43 ± 0.78% at 160 μg/mL) in comparison to apple pulp extract mediated AgNPs, while both AgNPs showed lower activity for DPPH (27.84 ± 0.56% and 13.12 ± 0.32% from cumin seed and apple pulp extracts, respectively). In summary, our results suggest the green non-cytotoxic AgNPs synthesized in this study could be a promising template for further biological and clinical applications. Graphical abstract