Abstract
AbstractThe study sought to investigate the impact of a holistic high-power microwave technology during all stages of the extraction on the quality, time of extraction, and degree of deacetylation (DD) of shrimp chitosan (SC) and crab chitosan (KC). The demineralization and deproteinization stages took 7 and 8 min, at 750 and 875 W, respectively. The deacetylation process was conducted at two powers, 875 W and 1250 W, for 10, 15, and 20 min. It only took 25 min at 875 W to successfully prepare chitosan with a high DD and 30 min to reach the maximum DD. The highest DDs by the potentiometric titration method, FTIR, and 1H NMR of SC were 86.6%, 86.7%, and 83%, compared to 83.8%, 82.7%, and 80% for KC, respectively. Extracted SC had 79% solubility, 14.125 kDa, a 46.57% crystallinity index, 705.40% WBC, and 434.60% FBC, against 74.5%, 16.982 kDa, 74.14%, 689.82%, and 413.20% for KC, respectively. The study proved that 30 min of holistic high-power microwave at 875 W produced low-molecular-weight chitosan with relatively high deacetylation and low content of viscosity, crystallinity, and protein residue. The technique can provide a feasible alternative to the commercial production of low-molecular-weight chitosan in less time and energy.
Publisher
Springer Science and Business Media LLC
Subject
General Earth and Planetary Sciences,General Physics and Astronomy,General Engineering,General Environmental Science,General Materials Science,General Chemical Engineering
Reference69 articles.
1. Sitohy M, Taha S, Osman A, Abdel-Hamid M, Hamed A, Abdelbacki A (2020) Antiviral action of native and methylated lactoferrin and β-lactoglobulin against potato virus Y (PVY) infected into potato plants grown in an open field. Antibiotics 9:430. https://doi.org/10.3390/antibiotics9070430
2. Sitohy M, Mahgoub S, Osman A (2011) Controlling psychrotrophic bacteria in raw buffalo milk preserved at 4 C with esterified legume proteins. LWT-Food Sci Technol 44:1697–1702. https://doi.org/10.1016/j.lwt.2011.03.008
3. Chobert JM, Sitohy M, Billaude S, Dalgalarrondo M, Haertlé T (2007) Anticytomegaloviral activity of esterified milk proteins and L-Polylysines. J Mol Microbiol Biotechnol 13:255–258
4. Sitohy M, Taha S, Abdel-Hamid M, Abdelbacki A, Hamed A, Osman A (2021) Protecting potato plants against PVX and PVY viral infections by the application of native and chemically modified legume proteins. J Plant Dis Prot 128:1101–1114. https://doi.org/10.1007/S41348-021-00448-9
5. Osman A, El-Didamony G, Sitohy M, Khalifa M, Enan G (2016) Soybean glycinin basic subunit inhibits methicillin resistant-vancomycin intermediate Staphylococcus aureus (MRSA-VISA) in vitro. Int J Appl Res Nat Prod 9:17–26