Abstract
AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.
Funder
Indian Council of Agricultural Research
Publisher
Springer Science and Business Media LLC
Subject
General Earth and Planetary Sciences,General Physics and Astronomy,General Engineering,General Environmental Science,General Materials Science,General Chemical Engineering
Reference35 articles.
1. Johnson KA, Johnson DE (1995) Methane emissions from cattle. J Anim Sci 73(8):2483–2492
2. Min B, Barry T, Attwood G, McNabb W (2003) The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review. Anim Feed Sci Technol 106(1):3–19
3. Dangal SR, Tian H, Zhang B, Pan S, Lu C, Yang J (2017) Methane emission from global livestock sector during 1890–2014: magnitude, trends and spatiotemporal patterns. Glob Change Biol 23(10):4147–4161
4. Steinfeld H, Gerber P, Wassenaar T, Castel V, Rosales M, De Haan C, LsL S (2006) Environmental issues and options. Food and Agriculture Organization, Rome
5. Tubiello F, Salvatore M, Cóndor Golec R, Ferrara A, Rossi S, Biancalani R, Federici S, Jacobs H, Flammini A (2014) Agriculture, forestry and other land use emissions by sources and removals by sinks. Statistics Division Food and Agriculture Organization, Rome
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