AFM study of complement system assembly initiated by antigen-antibody complex-Reference-Cited by-同舟云学术

AFM study of complement system assembly initiated by antigen-antibody complex

Published:2006-03-01 Issue:1 Volume:4 Page:194-206
ISSN:2391-5420
Container-title:Open Chemistry
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Author:

Ramanaviciene Almira,Snitka Valentinas¹,Mieliauskiene Rasa²,Kazlauskas Rolandas²,Ramanavicius Arunas

Affiliation:

1. 3Research Center for Microsystems and Nanotechnology, Kaunas University of Technology, Studentu 65, 3031, Kaunas, Lithuania

2. 1Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius 09, Lithuania

Abstract

AbstractThe shape and size of complement system C1 components assembled on a SiO2 surface after classical activation by antigen-antibody complex was determined by tapping mode atomic force microscopy (AFM). The SiO2 substrate was silanized and bovine leukemia virus proteins gp51 were covalently bound to the SiO2 substrate. Self-assembly of complement system proteins was investigated by AFM. Uniform coating of silanized surface by gp51 proteins was observed by AFM. After incubation of gp51 coated substrate in anti-gp51 antibody containing solution, Ag-Ab complexes were detected on the substrate surface by AFM. Then after treatment of Ag-Ab complex modified substrate by guinea-pig blood serum containing highly active complement system proteins for 3 minutes and 30 minutes features 2–3 times and 5–8 times higher in diameter and in height if compared with those observed after formation of Ag-Ab complex, were observed respectively on the surface of SiO2. This study revealed that AFM might be applied for the imaging of complement system assembly and provides valuable information that can be used to complement other well-established techniques.

Publisher

Walter de Gruyter GmbH

Subject

Materials Chemistry,General Chemistry

Link

https://www.degruyter.com/downloadpdf/journals/chem/4/1/article-p194.xml

Reference39 articles.

1. D. Fotiadis, S. Scheuring, S.A. Müller, A. Engel and D.J. Müller: “Imaging and manipulation of biological structures with the AFM”, Micron, Vol. 33, (2002), pp. 385–397.

2. S. Scheuring, F. Reiss-Husson, A. Engel, J.L. Rigaud and J.L. Ranck: “Highresolution AFM topographs of Rubrivivax gelatinosus light-harvesting complex LH2”, Embo J., Vol. 20, (2001), pp. 3029–3035.

3. H.J. Müller-Eberhard and P.A. Miescher (Eds.): Complement, Verlag, Berlin, 1985.

4. B. Walivaara, A. Askendal, I. Lundstrom and P. Tengvall: “Imaging of the early events of classical complement activation using antibodies and atomic force microscopy”, J. Coll. Interface. Sci., Vol. 187, (1997), pp. 121–127.

5. L. Mark, W.H. Lee, O.B. Spiller, D. Proctor, D.J. Blackbourn, B.O. Villoutreix and A.M. Blom: “The Kaposi's sarcoma-associated herpesvirus complement control protein mimics human molecular mechanisms for inhibition of the complement system”, J. Biol. Chem., Vol. 279, (2004), pp. 45093–45101.

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