Abstract
AbstractOzonation is a powerful technique to remove micropollutants from wastewater. As chemical oxidation of wastewater comes with the formation of varying, possibly persistent and toxic by-products, post-treatment of the ozonated effluent is routinely suggested. This study explored an enzymatic treatment of ozonation products using the laccase from Trametes versicolor. A high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC-HRMS) analysis revealed that the major by-products were effectively degraded by the enzymatic post-treatment. The enzymatic removal of the by-products reduced the ecotoxicity of the ozonation effluent, as monitored by the inhibition of Aliivibrio fischeri. The ecotoxicity was more effectively reduced by enzymatic post-oxidation at pH 7 than at the activity maximum of the laccase at pH 5. A mechanistic HPLC-HRMS and UV/Vis spectroscopic analysis revealed that acidic conditions favored rapid conversion of the phenolic by-products to dead-end products in the absence of nucleophiles. In contrast, the polymerization to harmless insoluble polymers was favored at neutral conditions. Hence, coupling ozonation with laccase-catalyzed post-oxidation at neutral conditions, which are present in wastewater effluents, is suggested as a new resource-efficient method to remove persistent micropollutants while excluding the emission of potentially harmful by-products.
Graphical abstract
Funder
European Regional Development Fund
Hochschule Niederrhein
Publisher
Springer Science and Business Media LLC
Subject
Health, Toxicology and Mutagenesis,Pollution,Environmental Chemistry,General Medicine
Reference68 articles.
1. Alder AC, Bruchet A, Carballa M, Clara M, Joss A, Löffler D, McArdell CS, Miksch K, Omil F, Tuhkanen T, Ternes TA (2006) Consumption and occurrence. In: Ternes TA and Joss A (ed) Human pharmaceuticals, hormones and fragrances. The challenge of micropollutants in urban water management, vol. 5. IWA Publishing, London, pp 15–54
2. Abejón R, de Cazes M, Belleville MP, Sanchez-Marcano J (2015) Large-scale enzymatic membrane reactors for tetracycline degradation in WWTP effluents. Water Res 73:118–131. https://doi.org/10.1016/j.watres.2015.01.012
3. Ahmed MB, Zhou JL, Ngo HH, Guo W, Thomaidis NS, Xu J (2017) Progress in the biological and chemical treatment technologies for emerging contaminant removal from wastewater: a critical review. J Hazard Mater 323(Pt A):274–298. https://doi.org/10.1016/j.jhazmat.2016.04.045
4. Alshabib M, Onaizi SA (2019) A review on phenolic wastewater remediation using homogeneous and heterogeneous enzymatic processes: current status and potential challenges. Sep Purif Technol 219:186–207. https://doi.org/10.1016/j.seppur.2019.03.028
5. Alygizakis NA, Gago-Ferrero P, Borova VL, Pavlidou A, Hatzianestis I, Thomaidis NS (2016) Occurrence and spatial distribution of 158 pharmaceuticals, drugs of abuse and related metabolites in offshore seawater. Sci Total Environ 541:1097–1105. https://doi.org/10.1016/j.scitotenv.2015.09.145
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