High-yield recombinant xylanase production by Aspergillus nidulans under pyridoxine limitation

Author:

Müller Michael12,Segato Fernando34,Prade Rolf A4,Wilkins Mark R2

Affiliation:

1. grid.425671.3 PFI Biotechnology Pirmasens Rhineland-Palatinate Germany

2. grid.65519.3e 0000000107217331 Department of Biosystems and Agricultural Engineering Oklahoma State University 224 Ag Hall 74078 Stillwater OK USA

3. grid.11899.38 0000000419370722 Department of Biotechnology University of São Paulo Lorena Brazil

4. grid.65519.3e 0000000107217331 Department of Microbiology and Molecular Genetics Oklahoma State University Stillwater OK USA

Abstract

Abstract The present study investigated the limitation of pyridoxine on an Aspergillus nidulans culture that produces xylanase B (XynB) as a client enzyme and was unable to synthesize pyridoxine. This technique was used to limit cell growth and divert substrate to product formation for a surface grown culture that could be used in trickle bed reactors. It was observed that growth was limited when pyridoxine was absent, while enzyme production was unaffected. Enzyme production was 1,026 U after 480 h of continuous fermentation, which was similar to a culture that grew on medium with pyridoxine. Furthermore, the present study investigated the growth rate of A. nidulans with pyridoxine in the medium and determined the productivity of XynB production with and without pyridoxine. A maximum growth rate of 0.311/h was observed. The maximum XynB productivity of 21.14 U/g h was achieved when pyridoxine was not added to the medium.

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Biotechnology,Bioengineering

Reference22 articles.

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