Affiliation:
1. grid.417587.8 0000000122433366 National Center for Toxicological Research, Division of Microbiology US Food and Drug Administration 3900 NCTR Road 72079-9502 Jefferson AR USA
2. grid.430387.b 0000000419368796 Department of Biology Rutgers, The State University of New Jersey New Brunswick NJ USA
3. grid.251990.6 0000000095628554 Department of Natural Sciences Albany State University Albany GA USA
4. grid.214458.e 0000000086837370 Department of Pediatrics and Communicable Diseases University of Michigan Ann Arbor MI USA
5. grid.417587.8 0000000122433366 Office of Pharmaceutical Science, Center for Drug Evaluation and Research US Food and Drug Administration Beltsville MD USA
Abstract
Abstract
Burkholderia cepacia complex (BCC) presence has been the cause of recalls of both sterile and non-sterile pharmaceutical products since these opportunistic pathogens have been implicated to cause infections to susceptible individuals. BCC are ubiquitous in nature, but in pharmaceutical settings the most common source is contaminated water systems. Some strains of BCC, previously described as Pseudomonas cepacia, were not readily detected by standard culture methods. We have explored different strategies to recover and enrich Burkholderia cenocepacia previously cultured in distilled water for 40 days. Enrichment media of varied nutrient concentrations and composition were used, including modified Tryptic Soy Agar or Broth (TSA or TSB), Reasoner’s 2nd Agar or Broth (R2A or R2AB), Brain–Heart Infusion Broth (BHIB), Mueller–Hinton Broth (MHB), and Ashdown’s (ASH) medium. Of the various broth media tested, cell growth was significantly greater in TSB and R2AB than in BHIB, MHB, or ASH broth. TSB and R2AB were also compared for their recovery efficiency. Generally, there was no significant difference between the numbers of B. cenocepacia grown on 15 differently modified TSA and five modified R2A solid media. Overall, however, diluted TSA and TSB media, and R2A and R2AB showed better recovery efficiency than TSA and TSB for inocula containing small numbers of cells. All strains persisted in distilled water for 40 days. Broth media were more effective than solid media for recovery of B. cenocepacia from distilled water. These results may assist in improving detection assays with recovery and enrichment strategies to maximize recovery of these fastidious organisms.
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
19 articles.
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