5-Aminolevulinic acid production from inexpensive glucose by engineering the C4 pathway in Escherichia coli-Reference-Cited by-同舟云学术

5-Aminolevulinic acid production from inexpensive glucose by engineering the C4 pathway in Escherichia coli

Published:2017-08-01 Issue:8 Volume:44 Page:1127-1135
ISSN:1476-5535
Container-title:Journal of Industrial Microbiology and Biotechnology
language:en
Short-container-title:

Author:

Ding Wenwen¹²,Weng Huanjiao¹²,Du Guocheng¹²,Chen Jian¹²,Kang Zhen¹²³

Affiliation:

1. 0000 0001 0708 1323 grid.258151.a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology Jiangnan University 214122 Wuxi People’s Republic of China

2. 0000 0001 0708 1323 grid.258151.a Synergetic Innovation Center of Food Safety and Nutrition Jiangnan University 214122 Wuxi Jiangsu China

3. 0000 0001 0708 1323 grid.258151.a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education Jiangnan University 214122 Wuxi People’s Republic of China

Abstract

Abstract 5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L. Following optimization of biosynthesis pathways towards coenzyme A and precursor (glycine and succinyl-CoA), and downregulation of hemB expression, the production of ALA was further increased to 2.81 g/L in batch-fermentation.

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Biotechnology,Bioengineering

Link

http://link.springer.com/article/10.1007/s10295-017-1940-1/fulltext.html

Reference46 articles.

1. Production of succinic acid by metabolically engineered microorganisms;Ahn;Curr Opin Biotechnol,2016

2. Recent advances in production of succinic acid from lignocellulosic biomass;Akhtar;Appl Microbiol Biotechnol,2014

3. Characterization of the Rhodobacter sphaeroides 5-aminolaevulinic acid synthase isoenzymes, HemA and HemT, isolated from recombinant Escherichia coli;Bolt;Eur J Biochem,1999

4. Directed evolution of enzymes for biocatalytic applications;Bornscheuer;Biocatal Biotransform,2001

5. AUU-to-AUG mutation in the initiator codon of the translation initiation factor IF3 abolishes translational autocontrol of its own gene (infC) in vivo;Butler;Proc Natl Acad Sci USA,1987

Cited by 48 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme;Metabolic Engineering;2024-09

2. Accelerated 5-aminolevulinic acid biosynthesis by coupling aconitase and ALA synthase in engineered Escherichia coli;Biochemical Engineering Journal;2024-09

3. Recent Advances in the Microbial Synthesis of Human Hemoglobin;Synthetic and Systems Biotechnology;2024-09

4. Improved fermentative gamma-aminobutyric acid production from glucose by the inactivation of respiratory chain components NDH-I and Cytbo₃ in Escherichia coli;Journal of Bioscience and Bioengineering;2024-09

5. Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production;Trends in Biotechnology;2024-08