A light-controlled cell lysis system in bacteria

Author:

Wang Geyi1,Lu Xin1,Zhu Yisha1,Zhang Wei1,Liu Jiahui1,Wu Yankang1,Yu Liyang1,Sun Dongchang1,Cheng Feng1

Affiliation:

1. 0000 0004 1761 325X grid.469325.f College of Biotechnology and Bioengineering Zhejiang University of Technology 310014 Hangzhou Zhejiang China

Abstract

Abstract Intracellular products (e.g., insulin), which are obtained through cell lysis, take up a big share of the biotech industry. It is often time-consuming, laborious, and environment-unfriendly to disrupt bacterial cells with traditional methods. In this study, we developed a molecular device for controlling cell lysis with light. We showed that intracellular expression of a single lysin protein was sufficient for efficient bacterial cell lysis. By placing the lysin-encoding gene under the control of an improved light-controlled system, we successfully controlled cell lysis by switching on/off light: OD600 of the Escherichia coli cell culture was decreased by twofold when the light-controlled system was activated under dark condition. We anticipate that our work would not only pave the way for cell lysis through a convenient biological way in fermentation industry, but also provide a paradigm for applying the light-controlled system in other fields of biotech industry.

Funder

the first-class discipline construction 10 program of Zhejiang Province.

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Biotechnology,Bioengineering

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