Affiliation:
1. grid.190737.b 0000000101540904 School of Chemistry and Chemical Engineering Chongqing University P.O. Box 94# Shazheng Street 174, Shapingba District 400044 Chongqing China
2. grid.213917.f 0000000120974943 School of Chemical and Biomolecular Engineering Georgia Institute of Technology 30332 Atlanta USA
Abstract
Abstract
In this study, a novel engineering Escherichia coli strain (CBMG111) with the expression of mgtCB gene was constructed for the enhanced fermentative production of succinic acid by utilizing the synergetic effect of mgtC gene to improve the growth of strains at the environment of low Mg2+ concentration and mgtB to enhance the transport of Mg2+ into cells. After the effect of the expression of the individual genes (mgtA, mgtB, mgtC) on the growth of E. coli was clarified, the fermentative production of succinic acid by CBMG111 was studied with the low-price mixture of Mg(OH)2 and NH3·H2O as the alkaline neutralizer and the biomass hydrolysates as the carbon sources, which demonstrated that the expression of mgtCB gene can significantly increase the productivity of succinic acid (2.97 g L−1 h−1) compared with that by using the engineering strain with the overexpression of mgtA gene.
Funder
National Natural Science Foundation of China
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
13 articles.
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