Affiliation:
1. grid.261331.4 0000000122857943 Department of Animal Sciences, Ohio State Agricultural Research and Development Center (OARDC) The Ohio State University 305 Gerlaugh Hall, 1680 Madison Avenue 44691 Wooster OH USA
2. grid.261331.4 0000000122857943 Renewable Energy Program, Agricultural Technical Institute The Ohio State University 1328 Dover Road 44691 Wooster OH USA
3. grid.261331.4 0000000122857943 Department of Chemistry and Biochemistry, Center for RNA Biology The Ohio State University 484 West 12th Avenue 43210 Columbus OH USA
Abstract
Abstract
Lignocellulose-derived microbial inhibitors (LDMICs) prevent efficient fermentation of Miscanthus giganteus (MG) hydrolysates to fuels and chemicals. To address this problem, we explored detoxification of pretreated MG biomass by Cupriavidus basilensis ATCC®BAA-699 prior to enzymatic saccharification. We document three key findings from our test of this strategy to alleviate LDMIC-mediated toxicity on Clostridium beijerinckii NCIMB 8052 during fermentation of MG hydrolysates. First, we demonstrate that growth of C. basilensis is possible on furfural, 5-hydroxymethyfurfural, cinnamaldehyde, 4-hydroxybenzaldehyde, syringaldehyde, vanillin, and ferulic, p-coumaric, syringic and vanillic acid, as sole carbon sources. Second, we report that C. basilensis detoxified and metabolized ~98 % LDMICs present in dilute acid-pretreated MG hydrolysates. Last, this bioabatement resulted in significant payoffs during acetone-butanol-ethanol (ABE) fermentation by C. beijerinckii: 70, 50 and 73 % improvement in ABE concentration, yield and productivity, respectively. Together, our results show that biological detoxification of acid-pretreated MG hydrolysates prior to fermentation is feasible and beneficial.
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
21 articles.
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