Selective bio-oxidation of propane to acetone using methane-oxidizing Methylomonas sp. DH-1

Author:

Hur Dong Hoon1,Nguyen Thu Thi1,Kim Donghyuk2,Lee Eun Yeol1

Affiliation:

1. 0000 0001 2171 7818 grid.289247.2 Department of Chemical Engineering Kyung Hee University 446-701 Yongin-si Gyeonggi-do Republic of Korea

2. 0000 0001 2171 7818 grid.289247.2 Department of Genetic Engineering Kyung Hee University 446-701 Yongin-si Gyeonggi-do Republic of Korea

Abstract

Abstract Propane is the major component of liquefied petroleum gas (LPG). Nowadays, the use of LPG is decreasing, and thus utilization of propane as a chemical feedstock is in need of development. An efficient biological conversion of propane to acetone using a methanotrophic whole cell as the biocatalyst was proposed and investigated. A bio-oxidation pathway of propane to acetone in Methylomonas sp. DH-1 was analyzed by gene expression profiling via RNA sequencing. Propane was oxidized to 2-propanol by particulate methane monooxygenase and subsequently to acetone by methanol dehydrogenases. Methylomonas sp. DH-1 was deficient in acetone-converting enzymes and thus accumulated acetone in the absence of any enzyme inhibition. The maximum accumulation, average productivity and specific productivity of acetone were 16.62 mM, 0.678 mM/h and 0.141 mmol/g cell/h, respectively, under the optimized conditions. Our study demonstrates a novel method for the bioconversion of propane to acetone using methanotrophs under mild reaction condition.

Funder

National Research Foundation of Korea

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Biotechnology,Bioengineering

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