Affiliation:
1. 0000 0001 2151 536X grid.26999.3d Graduate School of Pharmaceutical Sciences The University of Tokyo Hongo 7-3-1, Bunkyo-ku 113-0033 Tokyo Japan
2. 0000 0001 2151 536X grid.26999.3d Graduate School of Agricultural and Life Sciences The University of Tokyo Yayoi 1-1-1, Bunkyo-ku 113-8657 Tokyo Japan
3. 0000 0001 2151 536X grid.26999.3d Collaborative Research Institute for Innovative Microbiology, The University of Tokyo Yayoi 1-1-1, Bunkyo-ku 113-8657 Tokyo Japan
Abstract
Abstract
Bacterial secondary metabolites (SM) are rich sources of drug leads, and in particular, numerous metabolites have been isolated from actinomycetes. It was revealed by recent genome sequence projects that actinomycetes harbor much more secondary metabolite-biosynthetic gene clusters (SM-BGCs) than previously expected. Nevertheless, large parts of SM-BGCs in actinomycetes are dormant and cryptic under the standard culture conditions. Therefore, a widely applicable methodology for cryptic SM-BGC activation is required to obtain novel SM. Recently, it was discovered that co-culturing with mycolic-acid-containing bacteria (MACB) widely activated cryptic SM-BGCs in actinomycetes. This “combined-culture” methodology (co-culture methodology using MACB as the partner of actinomycetes) is easily applicable for a broad range of actinomycetes, and indeed, 33 novel SM have been successfully obtained from 12 actinomycetes so far. In this review, the development, application, and mechanistic analysis of the combined-culture method were summarized.
Funder
Japan Society for the Promotion of Science
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
54 articles.
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