Isolation and structure determination of a new lantibiotic cinnamycin B from Actinomadura atramentaria based on genome mining-Reference-Cited by-同舟云学术

Isolation and structure determination of a new lantibiotic cinnamycin B from Actinomadura atramentaria based on genome mining

Published:2016-08-01 Issue:8 Volume:43 Page:1159-1165
ISSN:1476-5535
Container-title:Journal of Industrial Microbiology and Biotechnology
language:en
Short-container-title:

Author:

Kodani Shinya¹²³,Komaki Hisayuki⁴,Ishimura Sho²,Hemmi Hikaru⁵,Ohnishi-Kameyama Mayumi⁵

Affiliation:

1. grid.263536.7 0000000106564913 College of Agriculture, Academic Institute Shizuoka University 836 Ohya, Suruga-ku 422-8529 Shizuoka Japan

2. grid.263536.7 0000000106564913 Graduate School of Integrated Science and Technology Shizuoka University 836 Ohya, Suruga-ku 422-8529 Shizuoka Japan

3. grid.263536.7 0000000106564913 Graduate School of Science and Technology Shizuoka University 836 Ohya, Suruga-ku 422-8529 Shizuoka Japan

4. grid.459867.1 0000000113716073 Biological Resource Center National Institute of Technology and Evaluation (NBRC) 2-5-8 Kazusakamatari, Kisarazu 292‒0818 Chiba Japan

5. grid.416835.d 0000000122220432 Food Research Institute NARO 2-1-12 Kan-nondai, Tsukuba 305-8642 Ibaraki Japan

Abstract

Abstract New lantibiotic cinnamycin B was isolated from the extract of Actinomadura atramentaria NBRC 14695T, based on genome mining and chemical investigation. The partial structure of cinnamycin B was established by 2D NMR experiments, which indicated that cinnamycin B had same methyl lanthionine bridging pattern with cinnamycin. The reduction with NaBH4-NiCl2 afforded the reduced cinnamycin B, and MS/MS experiment indicated the presence of hydroxy asparatic acid in the molecule. Cinnamycin B showed an antibacterial activity against Streptomyces antibioticus with dosage of 5 μg (0.5μL, 10 mg/mL solution) at spot-on-lawn testing method. The gene cluster of cinnamycin B on the genome of A. atramentaria was identified and discussed in comparison with that of cinnamycin.

Funder

Japan Society for the Promotion of Science

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Biotechnology,Bioengineering

Link

http://link.springer.com/content/pdf/10.1007/s10295-016-1788-9.pdf

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