Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK-Reference-Cited by-同舟云学术

Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK

Published:2013-04-01 Issue:3-4 Volume:40 Page:393-397
ISSN:1476-5535
Container-title:Journal of Industrial Microbiology and Biotechnology
language:en
Short-container-title:

Author:

Hou Lihua¹,Li Xiaoyang¹,Wang Cong¹,Cao Xiaohong¹,Wang Haiyong¹

Affiliation:

1. grid.419897.a 000000040369313X Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology Ministry of Education 300457 Tianjin People’s Republic of China

Abstract

Abstract An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes.

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Biotechnology,Bioengineering

Link

http://link.springer.com/content/pdf/10.1007/s10295-013-1241-2.pdf

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