Affiliation:
1. grid.22935.3f 0000000405308290 Center for Viticulture and Enology, College of Food Science and Nutritional Engineering China Agricultural University 100083 Beijing People’s Republic of China
2. grid.48166.3d 0000000099318406 College of Biology Science and Technology Beijing University of Chemical Technology 100029 Beijing People’s Republic of China
Abstract
Abstract
The genome-wide transcriptional responses of S. cerevisiae to heterologous carotenoid biosynthesis were investigated using DNA microarray analysis. The results show that the genes involved in metal ion transport were specifically up-regulated in the recombinant strain, and metal ions, including Cu2+, Fe2+, Mn2+, and Mg2+, were deficient in the recombinant strain compared to the ion content of the parent strain. The decrease in metal ions was ascribed to a decrease in cell membrane (CM) fluidity caused by lower levels of unsaturated fatty acids and ergosterol. This was confirmed by the observation that metal ion levels were restored when CM fluidity was increased by supplying linoleic acid. In addition, a 24.3 % increase in the β-carotene concentration was observed. Collectively, our results suggest that heterologous production of carotenoids in S. cerevisiae can induce cellular stress by rigidifying the CM, which can lead to a deficiency in metal ions. Due to the importance of CM fluidity in cellular physiology, maintaining normal CM fluidity might be a potential approach to improving carotenoid production in genetically engineered S. cerevisiae.
Funder
National Natural Science Foundation of China (CN)
National Natural Science Foundation of China
Chinese Universities Scientific Fund
China Agricultural University Dabeinong Education Fund
China Agriculture Research System
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
30 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献