Prospects for engineering dynamic CRISPR–Cas transcriptional circuits to improve bioproduction-Reference-Cited by-同舟云学术

Prospects for engineering dynamic CRISPR–Cas transcriptional circuits to improve bioproduction

Published:2018-07-01 Issue:7 Volume:45 Page:481-490
ISSN:1476-5535
Container-title:Journal of Industrial Microbiology and Biotechnology
language:en
Short-container-title:

Author:

Fontana Jason¹,Voje William E¹²,Zalatan Jesse G¹³,Carothers James M¹²

Affiliation:

1. 0000000122986657 grid.34477.33 Molecular Engineering and Sciences Institute and Center for Synthetic Biology University of Washington 98195 Seattle WA USA

2. 0000000122986657 grid.34477.33 Department of Chemical Engineering University of Washington 98195 Seattle WA USA

3. 0000000122986657 grid.34477.33 Department of Chemistry University of Washington 98195 Seattle WA USA

Abstract

Abstract Dynamic control of gene expression is emerging as an important strategy for controlling flux in metabolic pathways and improving bioproduction of valuable compounds. Integrating dynamic genetic control tools with CRISPR–Cas transcriptional regulation could significantly improve our ability to fine-tune the expression of multiple endogenous and heterologous genes according to the state of the cell. In this mini-review, we combine an analysis of recent literature with examples from our own work to discuss the prospects and challenges of developing dynamically regulated CRISPR–Cas transcriptional control systems for applications in synthetic biology and metabolic engineering.

Funder

Burroughs Wellcome Fund

Division of Molecular and Cellular Biosciences

University of Washington

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Biotechnology,Bioengineering

Link

http://link.springer.com/article/10.1007/s10295-018-2039-z/fulltext.html

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