Affiliation:
1. 0000 0001 0379 7164 grid.216417.7 School of Metallurgy and Environment Central South University 410083 Changsha Hunan People’s Republic of China
2. 0000 0001 0379 7164 grid.216417.7 School of Minerals Processing and Bioengineering Central South University 410083 Changsha Hunan People’s Republic of China
3. 0000 0001 0379 7164 grid.216417.7 Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution Central South University 410083 Changsha Hunan People’s Republic of China
4. 0000 0001 0379 7164 grid.216417.7 Key Laboratory of Biometallurgy of Ministry of Education Central South University 410083 Changsha People’s Republic of China
5. 0000 0000 9271 2478 grid.411503.2 College of Environmental Science and Engineering, Fujian Key Laboratory of Pollution Control and Resource Reuse Fujian Normal University 350007 Fuzhou Fujian Province People’s Republic of China
Abstract
Abstract
Extremely thermoacidophilic Crenarchaeota belonging to the order Sulfolobales, such as Metallosphaera sedula, are metabolically versatile and of great relevance in bioleaching. However, the impacts of extreme thermoacidophiles propagated with different energy substrates on subsequent bioleaching of refractory chalcopyrite remain unknown. Transcriptional responses underlying their different bioleaching potentials are still elusive. Here, it was first showed that M. sedula inocula propagated with typical energy substrates have different chalcopyrite bioleaching capabilities. Inoculum propagated heterotrophically with yeast extract was deficient in bioleaching; however, inoculum propagated mixotrophically with chalcopyrite, pyrite or sulfur recovered 79%, 78% and 62% copper, respectively, in 12 days. Compared with heterotrophically propagated inoculum, 937, 859 and 683 differentially expressed genes (DEGs) were identified in inoculum cultured with chalcopyrite, pyrite or sulfur, respectively, including upregulation of genes involved in bioleaching-associated metabolism, e.g., Fe2+ and sulfur oxidation, CO2 fixation. Inoculum propagated with pyrite or sulfur, respectively, shared 480 and 411 DEGs with chalcopyrite-cultured inoculum. Discrepancies on repertories of DEGs that involved in Fe2+ and sulfur oxidation in inocula greatly affected subsequent chalcopyrite bioleaching rates. Novel genes (e.g., Msed_1156, Msed_0549) probably involved in sulfur oxidation were first identified. This study highlights that mixotrophically propagated extreme thermoacidophiles especially with chalcopyrite should be inoculated into chalcopyrite heaps at industrial scale.
Funder
Postdoctoral Science Foundation of Central South University
National Natural Science Foundation of China
Hunan Provincial Science and Technology Department
Natural Science Foundation of Hunan Province
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
20 articles.
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