Affiliation:
1. grid.412022.7 0000000093895210 College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology Xin mofan Road 5 210009 Nanjing People’s Republic of China
2. National Engineering Technique Research Center for Biotechnology Nanjing People’s Republic of China
3. grid.454889.c State Key Laboratory of Motor Vehicle Biofuel Technology Nanyang People’s Republic of China
4. grid.412022.7 0000000093895210 State Key Laboratory of Materials-Oriented Chemical Engineering Nanjing People’s Republic of China
Abstract
Abstract
Although it is generally known that cofactors play a major role in the production of different fermentation products, their role has not been thoroughly and systematically studied. To understand the impact of cofactors on physiological functions, a systematic approach was applied, which involved redox state analysis, energy charge analysis, and metabolite analysis. Using uridine 5′-monophosphate metabolism in Saccharomyces cerevisiae as a model, we demonstrated that regulation of intracellular the ratio of NADPH to NADP+ not only redistributed the carbon flux between the glycolytic and pentose phosphate pathways, but also regulated the redox state of NAD(H), resulting in a significant change of ATP, and a significantly altered spectrum of metabolic products.
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献