Schisandrin B Inhibits Osteosarcoma Cell Proliferation and Promotes Apoptosis Through PI3K/AKT/mTOR Pathway Mediated Autophagy

Abstract

AbstractOsteosarcoma is the most common primary malignant bone tumor; the main treatment method is surgery and adjuvant chemotherapy, with a 5-year survival rate of less than 20% for metastatic patients. Schisandrin B is the most abundant and active ingredient found in the fruit of Schisandra chinensis (Turcz.) Baill., Schisandraceae, which has document properties such as liver protection, antioxidant, anti-inflammatory, antiaging, and antitumor. The present investigation explored the therapeutic effect of schisandrin B on osteosarcoma (MG63 cells). Cell proliferation and viability, scratch assay, and transwell migration analysis were used to detect the effects of schisandrin B on the growth activity, migration, and invasion of MG63 cells. The effects of schisandrin B on MG63 cell apoptosis were detected by flow cytometry and tunel staining. Western blot was used to detect the expression levels of autophagy and apoptosis related proteins. Immunofluorescence staining was used to detect schisandrin B effects of autophagy and apoptosis on MG63 cells. Schisandrin B inhibited the growth activity, migration ability, and invasion ability of osteosarcoma cells. In addition, schisandrin B induced apoptosis of MG63 cells through autophagy mediated by PI3K/AKT/mTOR signaling pathway. Graphical Abstract